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Gateway cloning is compatible with protein secretion from Pichia pastoris.
Esposito D, Gillette WK, Miller DA, Taylor TE, Frank PH, Hu R, Bekisz J, Hernandez J, Cregg JM, Zoon KC, Hartley JL. Esposito D, et al. Protein Expr Purif. 2005 Apr;40(2):424-8. doi: 10.1016/j.pep.2004.12.006. Protein Expr Purif. 2005. PMID: 15766886 Free PMC article.
RAS internal tandem duplication disrupts GTPase-activating protein (GAP) binding to activate oncogenic signaling.
Nelson AC, Turbyville TJ, Dharmaiah S, Rigby M, Yang R, Wang TY, Columbus J, Stephens R, Taylor T, Sciacca D, Onsongo G, Sarver A, Subramanian S, Nissley DV, Simanshu DK, Lou E. Nelson AC, et al. Among authors: taylor t. J Biol Chem. 2020 Jul 10;295(28):9335-9348. doi: 10.1074/jbc.RA119.011080. Epub 2020 May 11. J Biol Chem. 2020. PMID: 32393580 Free PMC article.
Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays.
Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W. Esposito D, et al. Among authors: taylor t. Protein Expr Purif. 2020 Oct;174:105686. doi: 10.1016/j.pep.2020.105686. Epub 2020 Jun 4. Protein Expr Purif. 2020. PMID: 32504802 Free PMC article.
Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays.
Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W. Esposito D, et al. Among authors: taylor t. bioRxiv [Preprint]. 2020 May 28:2020.05.27.120204. doi: 10.1101/2020.05.27.120204. bioRxiv. 2020. PMID: 32511418 Free PMC article. Updated. Preprint.
Uncovering a membrane-distal conformation of KRAS available to recruit RAF to the plasma membrane.
Van QN, López CA, Tonelli M, Taylor T, Niu B, Stanley CB, Bhowmik D, Tran TH, Frank PH, Messing S, Alexander P, Scott D, Ye X, Drew M, Chertov O, Lösche M, Ramanathan A, Gross ML, Hengartner NW, Westler WM, Markley JL, Simanshu DK, Nissley DV, Gillette WK, Esposito D, McCormick F, Gnanakaran S, Heinrich F, Stephen AG. Van QN, et al. Among authors: taylor t. Proc Natl Acad Sci U S A. 2020 Sep 29;117(39):24258-24268. doi: 10.1073/pnas.2006504117. Epub 2020 Sep 10. Proc Natl Acad Sci U S A. 2020. PMID: 32913056 Free PMC article.
2,979 results