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Protocol for cross-platform characterization of human and murine extracellular vesicles and particles.
Bojmar L, Kim HS, Sugiura K, Heissel S, Lucotti S, Cioffi M, Johnson KE, Cohen-Gould L, Zhang H, Molina H, Matei IR, Lyden D, Hoshino A. Bojmar L, et al. Among authors: hoshino a. STAR Protoc. 2024 Mar 15;5(1):102754. doi: 10.1016/j.xpro.2023.102754. Epub 2023 Dec 13. STAR Protoc. 2024. PMID: 38096060 Free PMC article.
Characterization of isolated extracellular vesicles and particles (EVPs) is crucial for determining functions and biomarker potential. Here, we present a protocol to analyze size, number, morphology, and EVP protein cargo and to validate EVP proteins in both humans and mic …
Characterization of isolated extracellular vesicles and particles (EVPs) is crucial for determining functions and biomarker potential. Here, …
Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids.
Bojmar L, Kim HS, Tobias GC, Pelissier Vatter FA, Lucotti S, Gyan KE, Kenific CM, Wan Z, Kim KA, Kim D, Hernandez J, Pascual V, Heaton TE, La Quaglia MP, Kelsen D, Trippett TM, Jones DR, Jarnagin WR, Matei IR, Zhang H, Hoshino A, Lyden D. Bojmar L, et al. Among authors: hoshino a. STAR Protoc. 2020 Dec 22;2(1):100225. doi: 10.1016/j.xpro.2020.100225. eCollection 2021 Mar 19. STAR Protoc. 2020. PMID: 33786456 Free PMC article.
We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, a …
We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (s …
Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation.
Zhang H, Freitas D, Kim HS, Fabijanic K, Li Z, Chen H, Mark MT, Molina H, Martin AB, Bojmar L, Fang J, Rampersaud S, Hoshino A, Matei I, Kenific CM, Nakajima M, Mutvei AP, Sansone P, Buehring W, Wang H, Jimenez JP, Cohen-Gould L, Paknejad N, Brendel M, Manova-Todorova K, Magalhães A, Ferreira JA, Osório H, Silva AM, Massey A, Cubillos-Ruiz JR, Galletti G, Giannakakou P, Cuervo AM, Blenis J, Schwartz R, Brady MS, Peinado H, Bromberg J, Matsui H, Reis CA, Lyden D. Zhang H, et al. Among authors: hoshino a. Nat Cell Biol. 2018 Mar;20(3):332-343. doi: 10.1038/s41556-018-0040-4. Epub 2018 Feb 19. Nat Cell Biol. 2018. PMID: 29459780 Free PMC article.
Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers.
Hoshino A, Kim HS, Bojmar L, Gyan KE, Cioffi M, Hernandez J, Zambirinis CP, Rodrigues G, Molina H, Heissel S, Mark MT, Steiner L, Benito-Martin A, Lucotti S, Di Giannatale A, Offer K, Nakajima M, Williams C, Nogués L, Pelissier Vatter FA, Hashimoto A, Davies AE, Freitas D, Kenific CM, Ararso Y, Buehring W, Lauritzen P, Ogitani Y, Sugiura K, Takahashi N, Alečković M, Bailey KA, Jolissant JS, Wang H, Harris A, Schaeffer LM, García-Santos G, Posner Z, Balachandran VP, Khakoo Y, Raju GP, Scherz A, Sagi I, Scherz-Shouval R, Yarden Y, Oren M, Malladi M, Petriccione M, De Braganca KC, Donzelli M, Fischer C, Vitolano S, Wright GP, Ganshaw L, Marrano M, Ahmed A, DeStefano J, Danzer E, Roehrl MHA, Lacayo NJ, Vincent TC, Weiser MR, Brady MS, Meyers PA, Wexler LH, Ambati SR, Chou AJ, Slotkin EK, Modak S, Roberts SS, Basu EM, Diolaiti D, Krantz BA, Cardoso F, Simpson AL, Berger M, Rudin CM, Simeone DM, Jain M, Ghajar CM, Batra SK, Stanger BZ, Bui J, Brown KA, Rajasekhar VK, Healey JH, de Sousa M, Kramer K, Sheth S, Baisch J, Pascual V, Heaton TE, La Quaglia MP, Pisapia DJ, Schwartz R, Zhang H, Liu Y, Shukla A, Blavier L, DeClerck YA, LaBarge M, Bissell MJ, Caffrey TC, Grandgenett PM, Ho… See abstract for full author list ➔ Hoshino A, et al. Cell. 2020 Aug 20;182(4):1044-1061.e18. doi: 10.1016/j.cell.2020.07.009. Epub 2020 Aug 13. Cell. 2020. PMID: 32795414 Free PMC article.
Metastasis: Lymphatic detours for cancer.
Hoshino A, Lyden D. Hoshino A, et al. Nature. 2017 Jun 28;546(7660):609-610. doi: 10.1038/546609a. Nature. 2017. PMID: 28658216 No abstract available.
Tumour exosomal CEMIP protein promotes cancer cell colonization in brain metastasis.
Rodrigues G, Hoshino A, Kenific CM, Matei IR, Steiner L, Freitas D, Kim HS, Oxley PR, Scandariato I, Casanova-Salas I, Dai J, Badwe CR, Gril B, Tešić Mark M, Dill BD, Molina H, Zhang H, Benito-Martin A, Bojmar L, Ararso Y, Offer K, LaPlant Q, Buehring W, Wang H, Jiang X, Lu TM, Liu Y, Sabari JK, Shin SJ, Narula N, Ginter PS, Rajasekhar VK, Healey JH, Meylan E, Costa-Silva B, Wang SE, Rafii S, Altorki NK, Rudin CM, Jones DR, Steeg PS, Peinado H, Ghajar CM, Bromberg J, de Sousa M, Pisapia D, Lyden D. Rodrigues G, et al. Among authors: hoshino a. Nat Cell Biol. 2019 Nov;21(11):1403-1412. doi: 10.1038/s41556-019-0404-4. Epub 2019 Nov 4. Nat Cell Biol. 2019. PMID: 31685984 Free PMC article.
Tumour exosome integrins determine organotropic metastasis.
Hoshino A, Costa-Silva B, Shen TL, Rodrigues G, Hashimoto A, Tesic Mark M, Molina H, Kohsaka S, Di Giannatale A, Ceder S, Singh S, Williams C, Soplop N, Uryu K, Pharmer L, King T, Bojmar L, Davies AE, Ararso Y, Zhang T, Zhang H, Hernandez J, Weiss JM, Dumont-Cole VD, Kramer K, Wexler LH, Narendran A, Schwartz GK, Healey JH, Sandstrom P, Labori KJ, Kure EH, Grandgenett PM, Hollingsworth MA, de Sousa M, Kaur S, Jain M, Mallya K, Batra SK, Jarnagin WR, Brady MS, Fodstad O, Muller V, Pantel K, Minn AJ, Bissell MJ, Garcia BA, Kang Y, Rajasekhar VK, Ghajar CM, Matei I, Peinado H, Bromberg J, Lyden D. Hoshino A, et al. Nature. 2015 Nov 19;527(7578):329-35. doi: 10.1038/nature15756. Epub 2015 Oct 28. Nature. 2015. PMID: 26524530 Free PMC article.
Pre-metastatic niches: organ-specific homes for metastases.
Peinado H, Zhang H, Matei IR, Costa-Silva B, Hoshino A, Rodrigues G, Psaila B, Kaplan RN, Bromberg JF, Kang Y, Bissell MJ, Cox TR, Giaccia AJ, Erler JT, Hiratsuka S, Ghajar CM, Lyden D. Peinado H, et al. Among authors: hoshino a. Nat Rev Cancer. 2017 May;17(5):302-317. doi: 10.1038/nrc.2017.6. Epub 2017 Mar 17. Nat Rev Cancer. 2017. PMID: 28303905 Review.
551 results