Solution structure of the 30 kDa N-terminal domain of enzyme I of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system by multidimensional NMR.
Garrett DS, Seok YJ, Liao DI, Peterkofsky A, Gronenborn AM, Clore GM.
Garrett DS, et al. Among authors: peterkofsky a.
Biochemistry. 1997 Mar 4;36(9):2517-30. doi: 10.1021/bi962924y.
Biochemistry. 1997.
PMID: 9054557
The active site His189 is located in a cleft at the junction of the alpha and alpha/beta domains and has a pKa of approximately 6.3. His189 has a trans conformation about chi1, a g+ conformation about chi2, and its Nepsilon2 atom accepts a hydro …
The active site His189 is located in a cleft at the junction of the alpha and alpha/beta domains and has a pKa of approximatel …