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Antibody elicited against the gp41 N-heptad repeat (NHR) coiled-coil can neutralize HIV-1 with modest potency but non-neutralizing antibodies also bind to NHR mimetics.
Nelson JD, Kinkead H, Brunel FM, Leaman D, Jensen R, Louis JM, Maruyama T, Bewley CA, Bowdish K, Clore GM, Dawson PE, Frederickson S, Mage RG, Richman DD, Burton DR, Zwick MB. Nelson JD, et al. Among authors: bewley ca. Virology. 2008 Jul 20;377(1):170-83. doi: 10.1016/j.virol.2008.04.005. Epub 2008 May 21. Virology. 2008. PMID: 18499210 Free PMC article.
Affinity maturation by targeted diversification of the CDR-H2 loop of a monoclonal Fab derived from a synthetic naïve human antibody library and directed against the internal trimeric coiled-coil of gp41 yields a set of Fabs with improved HIV-1 neutralization potency and breadth.
Gustchina E, Louis JM, Frisch C, Ylera F, Lechner A, Bewley CA, Clore GM. Gustchina E, et al. Among authors: bewley ca. Virology. 2009 Oct 10;393(1):112-9. doi: 10.1016/j.virol.2009.07.019. Epub 2009 Aug 19. Virology. 2009. PMID: 19695655 Free PMC article.
Binding of HIV-1 gp41-directed neutralizing and non-neutralizing fragment antibody binding domain (Fab) and single chain variable fragment (ScFv) antibodies to the ectodomain of gp41 in the pre-hairpin and six-helix bundle conformations.
Louis JM, Aniana A, Lohith K, Sayer JM, Roche J, Bewley CA, Clore GM. Louis JM, et al. Among authors: bewley ca. PLoS One. 2014 Aug 8;9(8):e104683. doi: 10.1371/journal.pone.0104683. eCollection 2014. PLoS One. 2014. PMID: 25105806 Free PMC article.
120 results