Endogenous C-terminal Tagging by CRISPR/Cas9 in Trypanosoma cruzi.
Lander N, Chiurillo MA, Vercesi AE, Docampo R.
Lander N, et al.
Bio Protoc. 2017 May 20;7(10):e2299. doi: 10.21769/BioProtoc.2299.
Bio Protoc. 2017.
PMID: 28758140
Free PMC article.
To achieve the C-terminal tagging of endogenous proteins in T. cruzi we use the Cas9/pTREX-n vector (Lander et al., 2015) to insert a specific tag sequence (3xHA or 3xc-Myc) at the 3' end of a specific gene of interest (GOI). ...Vectors pMOTag23M (Oberholzer et al., …
To achieve the C-terminal tagging of endogenous proteins in T. cruzi we use the Cas9/pTREX-n vector (Lander et al., 2015) to i …