Abstract
In this study, a loop-mediated isothermal amplification assay targeting the 16S-23S intergenic spacer regions (internal transcribed spacer) of Aeromonas caviae was developed. Eighteen reference strains and 109 clinical samples were analyzed. The results showed this detection technique is more reliable and convenient compared with common polymerase chain reaction and biochemical culture methods.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Aeromonas / genetics
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Aeromonas / isolation & purification*
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Intergenic / chemistry
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DNA, Intergenic / genetics
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Feces / microbiology*
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Gram-Negative Bacterial Infections / microbiology*
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Humans
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Molecular Diagnostic Techniques / methods*
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Molecular Sequence Data
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Nucleic Acid Amplification Techniques / methods*
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Sensitivity and Specificity
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Sequence Analysis, DNA
Substances
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DNA, Bacterial
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DNA, Intergenic